Methods and Techniques workshops
Samples preparation and data analysis in Electron Microscopy
Time: 10 pm - 2:30 am (+1) JST (Tokyo)/ 2 - 6:30 pm CET (Basel)/ 8 am - 1:30 pm EST (New York)/ 5 - 10:30 am PST (California)
Audience: WIA members
February 24th, 2022
From 14:00 till 18:30 CET [Basel time]
Technical workshop: Samples preparation and data analysis in Electron Microscopy
14:00-14:10: Brief introduction about the workshop
14:10-14:55: Eeva-Liisa Eskelinen: “Conventional Transmission Electron Microscopy (TEM), with a special focus on sample preparation and organelle morphology” (cultured mammalian cells)
14:55-15:40: Muriel Mari: “immuno-EM with a special focus on Tokuyasu procedure” (cultured mammalian cells and yeast)
15:40-16:00: Coffee break
16:00-16:45: Andreas Brech: “Correlative light and electron microscopy (CLEM)” (cultured mammalian cells)
16:45-17:30: Satoru Takahashi: “(CLEM) Array-Tomography” (cultured mammalian cells)
17:30-17:40: Coffee break
17:40-18:30: General discussion (EM and Autophagy: limitations and problems)
Methods to monitor macroautophagy in vitro and in vivo
9 pm - 1:30 am (+1) Tokyo
2 - 6:30 pm Basel
8 am - 1:30 pm New York
5 - 10:30 am San Diego
Expected duration: 4-5 hrs
Audience: WIA members
Thank you to all who registered and attended this fabulous event!
October 5th, 2021
This workshop focused on the methods and techniques to monitor macro-autophagy in different model systems.
Program (Times listed in CEST/GMT+2):
2:00-2:10: General introduction from the committee
2:10-2:55 : Willa Wen You Yim:
“Measuring autophagy flux in cultured mammalian cells with fluorescent protein-based probes”
2:55-3:40 : Rubén Gómez Sánchez:
“Monitoring macroautophagy in yeast”
3:40-4:00: Coffee break
4:00-4:45 : Jose L. Nieto Torres:
“Monitoring Canonical and Non-Canonical Autophagy in Mammalian Cells”
4:45-5:30 : Idil Orhon:
“Monitoring macroautophagy in 3D: in vivo and 3D culture”
5:30-5:40: Coffee break
5:40-6:30: General discussion (including last words from the committee)
Willa Yim is a final year PhD candidate in the laboratory of Noboru Mizushima in University of Tokyo, Japan. She has been studying the late stages of autophagy (autophagosome-lysosome fusion and lysosome activity during autophagy).
Dr. Rubén Gómez Sánchez is a biochemist interested in the molecular regulation of the autophagy machinery. During his PhD in Spain, he studied the role of the PINK1 protein in sensing mitochondrial damage and mediating the elimination of dysfunctional mitochondria by autophagy, in the laboratory of Dr. José Manuel Fuentes Rodríguez. In 2014, he joined to the laboratory of Prof. Fulvio Reggiori (The Netherlands) as a postdoctoral researcher. There, he started working on the molecular role of the Atg proteins during the autophagosome formation, using Saccharomyces cerevisiae as a cell model. Areas of expertise: autophagy, fluorescence microscopy, phagophore, autophagosome, membrane contact sites, Atg2.
Dr. Jose L. Nieto-Torres received his Ph.D. in Molecular Biology from the Autonomous University of Madrid, Spain, where he studied lethal human coronaviruses under the supervision of Professor Luis Enjuanes. Motivated by an interest in the involvement of aging, inflammation, and autophagy in coronavirus-related and other diseases, he joined Dr. Hansen's laboratory in 2016 as a postdoctoral fellow. Dr. Nieto-Torres studies the molecular mechanisms that regulate autophagy and plans to further explore their role in aging as an independent investigator.
Dr. Idil Orhon is a cell biologist focused on autophagy research on various physiological contexts. During her PhD in France she studies the interplay of autophagy and primary cilium and the role of this interplat in shear-stress induced kidney cells in the group of Prof. Patrice Codogno. She then completed her post-doc in the laboratory of Prof. Fulvio Reggiori in Netherlands in 2020 where she investigated the role of autophagy on the self-renewal of stem cells in the context of mouse salivary gland tissue and organoids.