Scientific Advice
Methods and Techniques workshops

Upcoming events

Methods to monitor selective autophagy in vitro and in vivo

Location: Zoom

Time:

TBA

Expected duration: 4-5 hrs

Audience: WIA members

January 2022

We will have our second methodological workshop in January, focusing on techniques to detect selective autophagy.

We will announce the date, time and program soon!

Previous events

Methods to monitor macroautophagy in vitro and in vivo

Location: Zoom

Time:

9 pm - 1:30 am (+1) Tokyo

2 - 6:30 pm Basel

8 am - 1:30 pm New York

5 - 10:30 am San Diego

Expected duration: 4-5 hrs

Audience: WIA members

Thank you to all who registered and attended this fabulous event!

October 5th, 2021

This workshop focused on the methods and techniques to monitor macro-autophagy in different model systems.

Program (Times listed in CEST/GMT+2):

2:00-2:10: General introduction from the committee 

2:10-2:55 : Willa Wen You Yim:  

“Measuring autophagy flux in cultured mammalian cells with fluorescent protein-based probes” 

2:55-3:40 : Rubén Gómez Sánchez:  

“Monitoring macroautophagy in yeast” 

3:40-4:00: Coffee break 

4:00-4:45 : Jose L. Nieto Torres:  

“Monitoring Canonical and Non-Canonical Autophagy in Mammalian Cells” 

4:45-5:30 : Idil Orhon:  

“Monitoring macroautophagy in 3D: in vivo and 3D culture” 

5:30-5:40: Coffee break  

5:40-6:30: General discussion (including last words from the committee)

Speakers Information

Willa Yim is a final year PhD candidate in the laboratory of Noboru Mizushima in University of Tokyo, Japan. She has been studying the late stages of autophagy (autophagosome-lysosome fusion and lysosome activity during autophagy).   

Dr. Rubén Gómez Sánchez is a biochemist interested in the molecular regulation of the autophagy machinery. During his PhD in Spain, he studied the role of the PINK1 protein in sensing mitochondrial damage and mediating the elimination of dysfunctional mitochondria by autophagy, in the laboratory of Dr. José Manuel Fuentes Rodríguez. In 2014, he joined to the laboratory of Prof. Fulvio Reggiori (The Netherlands) as a postdoctoral researcher. There, he started working on the molecular role of the Atg proteins during the autophagosome formation, using Saccharomyces cerevisiae as a cell model. Areas of expertise: autophagy, fluorescence microscopy, phagophore, autophagosome, membrane contact sites, Atg2. 

 

Dr. Jose L. Nieto-Torres received his Ph.D. in Molecular Biology from the Autonomous University of Madrid, Spain, where he studied lethal human coronaviruses under the supervision of Professor Luis Enjuanes. Motivated by an interest in the involvement of aging, inflammation, and autophagy in coronavirus-related and other diseases, he joined Dr. Hansen's laboratory in 2016 as a postdoctoral fellow. Dr. Nieto-Torres studies the molecular mechanisms that regulate autophagy and plans to further explore their role in aging as an independent investigator. 

 

Dr. Idil Orhon is a cell biologist focused on autophagy research on various physiological contexts.  During her PhD in France she studies the interplay of autophagy and primary cilium and the role of this interplat in shear-stress induced kidney cells in the group of Prof. Patrice Codogno.  She then completed her post-doc in the laboratory of Prof. Fulvio Reggiori in Netherlands in 2020 where she investigated the role of autophagy on the self-renewal of stem cells in the context of mouse salivary gland tissue and organoids.